Toxicology effects of δ-endotoxins and β-exotoxins of Bacillus thuringiensis in Wistar rats

Diouneia Lisiane Berlitz, Márcia Giovenardi, Lidia Mariana Fiuza

Abstract


Bacillus thuringiensis is an entomopathogen consisting of toxic proteins, such as the δ- endotoxins specific to insects. However, some subspecies can produce β-exotoxins that are nonspecifi c and toxic to the vertebrates. So, in this research the toxicology effect of two isolates of B. thuringiensis in Wistar rats have been assessed. In the in vivo assays the animals have been given 3.1010UFC/mL of B. thuringiensis thuringiensis and B. thuringiensis aizawai and supernatant bacterial suspensions orally. The excrements and the stomachic content of the animals have been collected and analyzed in SDS-PAGE (10%). The analyzed data show protein fragments between 151 kDa and 28 kDa, seeing that the fecal samples show only one track of peptides in the treatments when compared with the control group. The assessments of the stomach of the treated rats carried out under stereomicroscope have not shown alterations when compared with the control. These results indicate that the toxins which can be found in both B. thuringiensis species used in these assays can be degraded by the gastrointestinal conditions of the animals and have not presented oral toxic effect to this mammalian species under the conditions which the experiments had been carried out.

Key words: bacterium, toxins, mammalian, toxicity.

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